Possible Interaction: Asparagine and Glutamic Acid

“From atomistic simulations and analyses of water dynamics and hydrogen bond networks in cytochrome c oxidase, Ghane et al. (2018) present a compelling view of the coupling between the protonation state of a key glutamic acid residue and the conformation of an asparagine thought to function as a gate.”

“In the presence of glutamic acid , the glutamine and asparagine contents in the heart-perfusate system markedly increased; however, the ammonia content did not reduce significantly.”

“Virtual mutation and molecular dynamics simulations indicated that the introduction of Glu and Asn altered the interaction network at the − 3 subsite.”

“Here, we report that the substitution of an asparagine by a glutamic acid at position 45 of σG (N45E) strongly reduced binding by a previously characterized anti-sigma factor, CsfB (also known as Gin), in vitro, and increased the activity of σG in vivo.”

“Among them, only the Ala-220 ->Glu mutant completely suppressed the deleterious effect of the Asp-285- ->Asn mutation, indicating that position 220 is highly specific for the suppression.”

“By contrast, substituting alanine or asparagine amino acids at the two positions completely eliminates strand cleavage of substrate DNA, whereas substitution with glutamic acid markedly reduces the cleavage activity.”

“The products, L-glutamic acid and L-aspartic acid, showed competitive inhibition with respect to either L-glutamine or L-asparagine as substrates.”

“Repression of the biosynthesis is less if glutamic acid is added in mixtures with proline, beta-alanine and asparagine.”