Possible Interaction: Ascorbate and Ferric Ion

“ Ascorbate may be able to reduce the R183E ferric and ferrous dioxygen complexes in active site conformations that cannot be reduced by NADPH-cytochrome P450 reductase.”

“The two types of substitution had similar effects on ferritin functionality: (i) they … decreased ferroxidase activity by about 50%; (ii) they inhibited iron incorporation from Fe(III) … ascorbate ; (iii) they resulted in loss of Fe and Tb binding sites; … inhibition of iron oxidation by Tb (but not by Zn).”

“ Ascorbate and either soluble complexes of Cu(II) or Fe(III) provoked changes in red cell morphology, alteration in the polypeptide pattern of membrane proteins, and significant increases in methemoglobin.”

“We describe an automated method for determining ascorbate in serum and urine by using the reduction of ferric iron by ascorbate and the formation of a color between the resulting ferrous iron and Ferrozine [3-(2-pyridyl)-5,6-bis(4-phenylsulfonic acid)-1,2,4-triazine].”

“The recovery of sensitivity to H2O2 by ascorbate is completely inhibited by 1,10-phenanthroline, indicating that ascorbate is mediating the reduction of Fe(III).”

“Ferricyanide inhibited iron uptake, while ferrocyanide and ascorbate increased the uptake of Fe(III) but not Fe(II).”

“which in turn reacts with Tyr-OH to give Tyr-O.. In the presence of ascorbate , added to reduce the monomeric Fe(III) formed, 80% of the Fe(II) added produced a radical.”

“ Ferric iron is inactive unless ascorbate is included to reduce it.”

“The rate of lipid peroxidation (LPO) in rat liver mitochondria induced by the Fe++ + ascorbate system was considerably reduced in 1--12-month-old rats, remained unchanged thereafter up to the 24th postnatal month and was decreased in hyperthyroid rats aged 1- and 3 months.”

“At 10(-4) M, Fe(II) and Fe(III) strongly enhanced the chromosome-damaging capacity of ascorbate.”

“Fe(II) and Fe(III) (10(-4) or 10(-5) M) reduced or abolished the mitosis-inhibiting action of ascorbate.”