Possible Interaction: Arachidonic Acid and Phosphatidic Acid

“In addition to phosphatidylinositol phosphatidylcholine, phosphatidylethanolamine and phosphatidic acid may contribute to arachidonic acid release.”

“ AA failed to stimulate [14C]SA incorporation into PtdIns precursors, namely phosphatidic acid and cytidinediphosphodiacylglycerol; furthermore, enhanced [14C]SA incorporation, brought about by exogenously administered AA, was not restricted to PtdIns tetraenoic species.”

“Taken together, the combined levels of PEt and PA (an end product of phopholipase D, PLD) were about 42-fold higher than the level of AA at 5-10 min.”

“ PA also stimulated release of arachidonic acid from keratinocytes.”

“The present study suggests that bradykinin, histamine, and phosphatidic acid may interfere with the distribution and release of arachidonic acid in human keratinocytes in culture.”

“However, arachidonic and stearic acids were less abundant in phosphatidic acid (PtdA) and diacylglycerols in non-stimulated platelets.”

“Higher concentrations of AA (0.5-50 microM) stimulate the formation of a further amount of PA (200-250%), and phosphorylation of 40,000 molecular weight protein, platelet aggregation, and serotonin release.”

“A detailed analysis of the fatty acid composition … amount of unsaturated fatty acids was increased in PA from stimulated cells concomitantly with … saturated ones; in particular, arachidonic acid was increased approximately 2-fold only 2 min after con A … acid was decreased for the whole period investigated (20 min).”

“The addition of arachidonic acid causes little (less than 20%) production of phosphatidic acid.”

“In rat hepatocytes, arachidonic acid inhibited 32P incorporation into phosphatidylinositol and phosphatidic acid without any significant effect on 32P incorporation into phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine.”