supp.ai
Discover Supplement-Drug Interactions
Disclaimer: The information contained herein should NOT be used as a substitute for the advice of an appropriately qualified and licensed physician or other health care provider. The tool is not a substitute for the care provided… (more)
Last Updated: 3 years ago
supplement:
Arachidonic Acid
Research Papers that Mention the Interaction
“ GnRH stimulates arachidonic acid (AA) release from LbetaT2 gonadotrope cells via the Ca(2+)-independent phospholipase A(2) (iPLA(2)) and not via the more common Ca(2+)-dependent cytosolic phospholipase A(2)alpha (cPLA(2)alpha).”
Elucidation of mechanisms of the reciprocal cross talk between gonadotropin-releasing hormone and prostaglandin receptors.“ Arachidonic acid and 5-HETE potentiated GnRH-induced LH release up to 249% and 429%, respectively, when applied in parallel with GnRH (1 nmol/l) as a 4-min pulse at a concentration of 10 pmol/l.”
Inhibitory effect of leukotrienes on luteinizing hormone release.“ LHRH induced the release of [3H]arachidonic acid ([3H]AA) from [3H]AA-prelabelled anterior pituitary tissue from pro-oestrous rats; a response which was sensitive to inhibitors of PLA2, of protein kinase C (PKC) and of protein synthesis.”
“ GRF (10(-10) M) stimulated both AA and SRIF release significantly within 20 min, with maximum release occurring at 30 min.”
“Combined treatment with AA and LHRH or AA plus TPA showed additive effects on P formation.”
“However, when AA (10(-5) M) was added during the last 6 h to the cultures containing LHRH and FSH, AA partially reversed the inhibitory action of LHRH (by 42%).”
“Taken together, these results further support the hypothesis that AA (or its metabolites) play a stimulatory role in the direct action of LHRH on ovarian steroidogenesis.”
“In rat granulosa cells prelabeled with [3H]arachidonic acid, addition of LHRH , A23187 or 12‐0‐tetradecanoyl phorbol‐13‐acetate (TPA) enhanced the release of [3H]arachidonic acid into the culture medium.”
“These results suggest that the stimulatory effect of LHRH on arachidonic acid release is coupled more tightly to a Ca2+‐dependent rather than a protein kinase C‐mediated pathway.”
“Maximal or near-maximal concentrations of arachidonic acid or the phorbol ester, 12-O-tetradecanoylphorbol 13-acetate, were less effective than a maximal dose of GnRH in stimulating LH release.”
“In pre-labelled, superfused pituitary cells, GnRH dose-dependently enhanced the release of [3H]arachidonic acid , which occurred simultaneously with the secretion of luteinizing hormone (LH).”
“Release of LHRH, however, was clearly stimulated when the lipoxygenase inhibitor nordihydroguaiaretic acid was added to the medium, suggesting that some arachidonic acid metabolites are inhibitory to LHRH secretion.”
“In cells prelabeled with [3H]arachidonic acid, LHRH caused an increase in the level of [3H]arachidonic acid released in the culture medium, to 125-150% of control levels at the end of a 60-min incubation period.”
“In subsequent time-course and dose-response experiments, a significant effect on [3H]arachidonic acid release could be observed as early as 15 min after LHRH addition, and the lowest effective dose was 10(-8) M LHRH.”
Help us improve supp.ai, we'd love to hear your feedback: Submit Feedback