Possible Interaction: Arachidonic Acid and Glutathione

“Here, we report that GSH promotes concomitant formation of the current oxidative stress biomarkers malondialdehyde (MDA) and 15(S)‐8‐iso‐prostaglandin F2α from AA via PGHS.”

“Preincubation with low doses of AA increased glutathione ( GSH ) levels as well as GCLC and GCLM mRNAs, and this protected against H2O2 and menadione toxicity.”

“The inclusion of reduced glutathione in incubations containing p-[14C] phenetidine, rabbit kidney medulla microsomes and arachidonic acid resulted in a decrease in radioactivity bound to protein and an increase in radioactivity in the aqueous phase after extraction.”

“HG and AA synergistically decreased cell viability and increased glutathione (GSH) oxidation and lipid peroxidation.”

“ AA inhibits at lower concentrations in the absence of glutathione (I50=15 µM) than in the presence of glutathione (I50=42 µM).”

“Although total conversion of AA through the lipoxygenase pathway is lowered by GSH depletion, significant 12-HETE formation was observed suggesting that GSH and GSH-Px are not required for the generation of 12-HETE in human platelets.”

“In platelets from control subjects, the GSH level decreased to 21% of the initial level within 30 sec in response to arachidonic acid (1.65 mM) and rapidly recovered to 91% by 1 min.”

“These results suggested that colon carcinogenesis enhanced by beef tallow was partly caused by oxidative stress and arachidonic acid cascade , which were reduced by GSH.”

“ AA elevated the glutathione level by 52 and 14% and 4-hydroxynonenal by 63 and 39% in normoxia and ischemia, respectively.”

“Surprisingly, intracellular glutathione GSH , 20 mm) enhanced the effect of AA on maximal IA (−62%) and with AA slowed inactivation of IA.”