Possible Interaction: Arachidonic Acid and Glucose

“These effects of AA were associated with significant decreases in the cellular consumption of D-glucose and L-alanine as well as decreased rates of production of nitric oxide and ammonia.”

“Also, platelets incubated with arachidonic acid and a high concentration of glucose increased HETE production, an effect predicted by the capacity of glucose to act as an OH .”

“These results suggest that the inhibitory effect of the arachidonic acid on leptin secretion and expression may be due, al least in part, to the increase in the anaerobic utilization of glucose.”

“AA-induced insulin secretion was augmented in islets from obese compared with lean rats at high concentrations of AA in the presence of 3.3 mM glucose.”

“Twenty‐five millimoles of glucose , not mannitol or L‐glucose, significantly increased the [3H]‐arachidonic acid (AA) release compared to control.”

“Nitric oxide-induced release of [3H]arachidonic acid possessed an obligatory requirement for glucose, was highly correlated with the concentration of glucose in the medium, and was dependent on the metabolism of glucose.”

“Insulin secretagogues, such as glucose and the muscarinic agonist carbachol, stimulate arachidonic acid accumulation, although the mechanisms involved are controversial: carbachol is believed to stimulate phospholipase A2, while glucose-induced arachidonic acid release is the result of diacylglycerol hydrolysis [Konrad, R. J., et al. (1992) Biochem.”

“However, phosphorylation of an 80 kD protein substrate of protein kinase C was not increased when intact … insulin secretion seen with arachidonic acid were not mediated by protein kinase C. These data suggest that arachidonic acid … glucose may influence insulin secretion by inhibiting … effect on protein kinase C activity.”

“ Arachidonic acid potentiated [3H]InsP accumulation in response to low concentrations of glucose.”

“The cyclooxygenase inhibitors indomethacin, ibuprofen, and eicosatetraynoic acid potentiated [3H]InsP production in response to 5 and 10 mM glucose but not to 17 mM glucose.”

“The data indicate that glucose may play an important role in the regulation of release and metabolism of arachidonic acid after agonist stimulation.”