Possible Interaction: Arachidonic Acid and Estradiol

“This response to estradiol was clearly dose-dependent, and it was further enhanced by addition of arachidonic acid , the precursor for prostaglandin synthesis, to the medium.”

“In this study, treatment of steroid-starved ERα-positive MCF-7 and T47D mammary carcinoma cells with 17β-estradiol resulted in increased cellular uptake of the PUFA arachidonic acid ( AA ) and eicosapentaenoic acid (EPA), important building blocks for cellular membranes, and increased ACSL4 protein levels.”

“Treatment of macrophages with 17-β estradiol elicited an increased arachidonic acid (AA) release and an up-regulation of both cyclooxygenesis-1 and cyclooxygenasis-2 enzymes at both the transcript and protein levels.”

“… beta-estradiol at 2 different concentrations (10(-…) in the presence of arachidonic acid for various times (5 min up to 24 h) effected an increase in the production of PGI2 (measured … F1 alpha) of 36-47% in the artery and 16-21% in the vein over basal values (100%).”

“ Arachidonic acid release was increased in the presence of oestradiol sulphate , oestrone and oestradiol but reduced by oestrone sulphate, dehydroepiandrosterone sulphate, progesterone, dehydroepiandrosterone and, to a lesser extent, by pregnenolone sulphate and testosterone sulphate.”

“Pretreatment with 17 beta-estradiol stimulated the LH responses to LHRH, TPA, and low concentrations of AA.”

“The present findings suggest that oestradiol , at a relatively high concentration, may interfere with the access of arachidonic acid to the cyclo-oxygenase enzyme.”

“We report that arachidonic acid can reverse the inhibition of masculine development in male embryos produced by estradiol-17 beta or by cyproterone acetate, an androgen receptor-site blocker, and that such reversal can be prevented by an inhibitor of cyclooxygenase, such as indomethacin.”

“In the thrombocytes, 17β-estradiol decreased aggregatory response to arachidonic acid and synthesis of thromboxane B2.”