Possible Interaction: Arachidonic Acid and Epoprostenol

“In vitro 9 beta-methyl carbacyclin and epoprostenol inhibited platelet aggregation induced by ADP, collagen, the endoperoxide analogue U46619 and arachidonic acid.”

“The studies first demonstrate that a single protein with three … Km of approximately 3.2 microM. Specific upregulation of PGI2 biosynthesis through expression of the engineered … inhibiting platelet aggregation induced by AA in vitro, which creates a great potential for the … new therapeutic interventions for strokes and heart attacks.”

“The substance has been shown to modulate arachidonic acid metabolism by enhancing the production and release of prostacyclin and prostaglandin E2 from tissues and whole blood, and inhibiting leukotriene B4 generation in leukocytes.”

“Inhibition occurred in the human cultures when PGI2 formation was elicited with arachidonic acid , ionophore A23187 or thrombin.”

“Treatment of the WPS with prostacyclin (PGI2) or iloprost prior to stimulation by AA resulted in a dose-dependent inhibition of the 5-HT-mediated contraction, due to inhibition of 5-HT release.”

“The discovery of prostacyclin has given a new insight into arachidonic acid metabolism and has led to a new hypothesis about mechanisms of haemostasis.”

“Administration of arachidonic acid (AA) (45 micrograms) to the system led to a further increase (eight- to ninefold) of PGI2 and yielded marked thromboxane formation (20-25 ng/ml).”

“The discovery of prostacyclin has given a new insight into arachidonic acid metabolism and has led to a new hypothesis about mechanisms of haemostasis.”

“These labile metabolites of AA antagonize each other: thromboxane A2 is a vasoconstrictor and proaggregatory agent, whereas prostacyclin dilates arteries, prevents platelets from aggregation, and dissipates the preformed platelet clumps.”

“ PGI2 dose dependently inhibits platelet activation induced by adenosine-5′-diphosphate, arachidonic acid , collagen, and low-dose thrombin.”