Possible Interaction: Arachidonic Acid and Diclofenac

“As a non-steroidal anti-inflammatory drug (NSAID), DS binds to both forms of COX (COX-1 and COX-2) and inhibits the conversion of arachidonic acid into pro-inflammatory prostaglandins by means of chelation.”

“In vitro, arachidonic acid and diclofenac synergistically induced neuroblastoma cell death.”

“In anesthetized rats treated with bacterial lipopolysaccharide (LPS) to induce the expression … marked increase in PGE2 production that followed bolus intravenous injection of arachidonic acid (3 mg kg−… inhibited by diclofenac but largely unaffected by the COX-2-selective inhibitor …- dimethyl-3-(2-propoxy)-4-methanesulfonylphenyl)-2(5H)-furanone).”

“Second, we show that supplying arachidonic acid to increase prostanoid production reduces the effectiveness of both currently used nonsteroidal antiinflammatory drugs (NSAIDs) ( diclofenac ) and novel COX‐2‐selective inhibitors (NS‐398, celecoxib) as inhibitors of COX‐2 activity.”

“ Diclofenac , the first nonsteroidal anti-inflammatory agent (NSAID) to be approved that is a phenylacetic acid derivative, competes with arachidonic acid for binding to cyclo-oxygenase, resulting in decreased formation of prostaglandins.”

“Instead, by enhancing its reincorporation into triglycerides, diclofenac sodium reduces the intracellular level of free arachidonic acid.”

“ Diclofenac sodium acts by potent cyclo-oxygenase inhibition, reduction of arachidonic acid release , and enhancement of arachidonic acid uptake.”

“Discussion: Research suggests diclofenac can inhibit the thromboxane-prostanoid receptor, affect arachidonic acid release and uptake, inhibit lipoxygenase enzymes, and activate the nitric oxide–cGMP antinociceptive pathway.”

“ Diclofenac , along with several others NSAIDs and cyclooxygenase inhibitors, depressed aggregation produced by arachidonic acid in platelet rich plasma from healthy volunteers.”

“ Diclofenac , a phenylacetic acid derivative, is a potent inhibitor of cyclooxygenase enzyme activity, and may also interact with the lipoxygenase enzyme pathway, and with the release and reuptake of arachidonic acid.”