Possible Interaction: Arachidonic Acid and Bradykinin

“The non-selective inhibitor of arachidonic acid metabolism eicosatetraynoic acid inhibits the EDHF-mediated component of the relaxation to bradykinin.”

“From experiments dealing with the vasomotor effects Ellis et al. (Am J Physiol 255: H397-H400, 1988) concluded an interaction of BK and AA in brain injury.”

“ Bradykinin induces an increment in intracellular cyclic AMP concentrations of human synovial fibroblasts and evokes the release of [3H] arachidonic acid and [3H]-E prostaglandins from human synovial fibroblasts prelabeled in their phospholipids.”

“In this model, bradykinin (BK) increases the release of arachidonic acid ( AA ), the precursor of labor-promoting prostaglandins.”

“In cultured decidua-derived cells, bradykinin stimulates the release of arachidonic acid , interleukin-6 (IL-6), and interleukin-8 (IL-8).”

“ BK and IL-1 beta both significantly stimulated the release of [3H]arachidonic acid.”

“These data show that BK via BK-B2 receptors, as well as thrombin, have the capacity to stimulate arachidonic acid release and subsequent prostanoid biosynthesis in human PDL-cells and thus may be implicated in the tissue reactions involved in orthodontic tooth movement.”

“ Bradykinin and kallidin stimulated a dose and time-dependent release of arachidonic acid from stromal cells which, with 100 nmol/L bradykinin, was 30-150% above basal release and maximal at 5 min.”

“Activation of arachidonic acid release by bradykinin was potentiated by interleukin-1 added either simultaneously with bradykinin or to cultures 24 h before addition of bradykinin.”

“Exposure of human synovial fibroblasts prelabelled with [3H]arachidonic acid to bradykinin causes a rapid and sustained increase in arachidonic acid release, a transient increase in cytosolic calcium and an increase in radiolabelled diacylglycerol.”

“ Bradykinin (100 nmol/L) … release of arachidonic acid (within 2 min) associated with an increase in inositol trisphosphate which was detectable after 20 s. Protein kinase C activation by … enhanced arachidonic acid release in response to both bradykinin and the Ca++ ionophore A23187 but inhibited bradykinin-stimulated phosphoinositide hydrolysis.”