Possible Interaction: Arabinose and Glucose

“ResultsAddition of l-arabinose reduced the production rate of d-glucose compared to the rates measured in the absence of l-arabinose for several hours in vitro.”

“A mechanism termed ‘catabolite repression’ has evolved to repress genes for the catabolism of lactose, arabinose and other sugars in the presence of glucose , even when these secondary sugars are also present (for review, see Gancedo, 1992).”

“A synergistic effect of combining sugars was observed when a mixture of sugars consisting of glucose and arabinose , or glucose and xylose, was applied: the displayed concentrations were consistently higher than was expected on the basis of the individual calibration curves.”

“Xylose transport activity in strain D required induction, while arabinose permease synthesis did not require inducer but was subject to repression by glucose.”

“Competitive inhibition of L-arabinose transport by glucose and countertransport of L-arabinose in the presence of glucose suggest that L-arabinose and glucose share the same transport system.”

“ L-Arabinose exhibits some inhibitory action on the effect of glucose and at 15′ after glucose + insulin.”

“Therefore, in the presence of D-glucose , utilization of D-xylose and L-arabinose is poor as the Hxt transporters prefer D-glucose.”

“The presence of d-glucose is thus preventing the fermentation of l-arabinose in S. cerevisiae DS61180.”

“ l-Arabinose-induced GAL2 expression was also subject to catabolite repression by d-glucose.”

“These results suggest that overexpression of pyruvate kinase in the araR deletion strain increased the specific consumption rate of l-arabinose and that citrate synthase activity becomes a new bottleneck in the engineered pathway during the simultaneous utilization of d-glucose and l-arabinose.”

“Inhibition of l-arabinose transport by the most abundant sugars in hydrolysates, d-glucose and d-xylose was far less pronounced than observed with Gal2.”