Possible Interaction: Antioxidants and Serotonin

“In colon epithelial …), direct application of 5-HT induced production of reactive oxygen species (ROS) and monocyte-epithelial adhesion, an … not only by 5-HT receptor antagonists (tropisetron, RS39604, and SB269970), antioxidants (ascorbic acid, apocynin), and various inhibitors of NADPH oxidase (… (PDTC), but also by transfection with Nox2 siRNA.”

“1) Antioxidants inhibit activation of ERK by 5-HT. (”

“Abstract The widely used antioxidant ascorbate decreases specific 5-HT binding to nerve ending membrane preparations isolated from human or rat brain.”

“Migration blockage is mediated by reactive oxygen species (ROS) generation since antioxidants , such as Vit C and Cu-Zn superoxide dismutase, prevent the inhibitory effects of 5-HT on cell migration.”

“They support a …, because 1) chemical antioxidants … 5-HT signal, 2) oxidants and 5-HT selectively activate ERK to a similar degree, 3) 5-HT produces superoxide and H(… [NAD(P)H oxidase] has been implicated as the source of the ROS, which react selectively downstream of classical PKC.”

“We presently show … 1 microM 5-HT also rapidly elevates phosphorylation and activation of the mitogen-activated protein (MAP) kinases extracellular signal-regulated kinase (ERK) 1 and ERK2 of SMCs, and the enhanced phosphorylation is blocked by the antioxidants Tiron, N-acetyl-L-cysteine (NAC), and Ginkgo biloba extract.”

“We presently show … 1 μM 5-HT also rapidly elevates phosphorylation and activation of the mitogen-activated protein (MAP) kinases extracellular signal-regulated kinase (ERK) 1 and ERK2 of SMCs, and the enhanced phosphorylation is blocked by the antioxidants Tiron, N-acetyl-l-cysteine (NAC), and Ginkgo biloba extract.”