Possible Interaction: Antioxidants and Buthionine Sulfoximine

“The activity of CPCD in HL-60 and K562 cells was modulated by glutathione (GSH), since depletion of this intracellular thiol-based antioxidant with buthionine sulfoximine resulted in significantly ( p < 0.05) greater potency in antiproliferation assays.”

“Exposure of HaCaT cells with BSO leads to altered expression of antioxidant genes and proteins, i.e., thioredoxin reductase (TrxR) and peroxiredoxin 6 (Prx6) whereas, in our study, pretreatment of PEGylated CeNPs reduces the need for induction of genes that produce enzymes involved in the defense against oxidative stress.”

“The antioxidant system can partially compensate the damage induced by BSO plus 1,25(OH)2D3.”

“The cell’s antioxidant defences can be compromised by, for example, inhibiting catalas0 activity by 3-aminotriazole or depleting glutathione levels using buthionine sulphoximine.”

“Also, BSO treatment increased the activity of phase II antioxidant enzyme, NAD(P)H: quinone oxidoreductase-1 (NQO-1).”

“As these changes may depend on the oxidative status, we administrated buthionine sulfoximine (BSO) to decrease the antioxidant capacity of some birds.”

“ BSO exposure resulted in several detrimental effects, decreasing glutamate-cysteine ligase (GCL) activity, cystine uptake, GSH intracellular content and the activities of the antioxidant enzymes glutathione peroxidase (GPx) and glutathione reductase (GR).”

“Results: Inhibition of GSH production by BSO enhanced CD36 translational efficiency to induce CD36 protein expression and lipid accumulation that was blocked by antioxidant (enzyme).”

“ Antioxidants such as trolox or N-acetyl cysteine prevented this synergistic toxicity of SAMe plus AA or SAMe plus BSO , respectively.”

“The translocation of PKC-epsilon by BSO was blocked by antioxidant trolox , suggesting the PKC-epsilon as a downstream of reactive oxygen species (ROS) elevated by BSO.”