Possible Interaction: Aminoglutethimide and Progesterone

“ Aminoglutethamide blocked progesterone secretion and increased the number of cells present after 3 days of culture.”

“In accordance with in vitro studies, the inhibitory effect of AG on placental progesterone formation is due to an inhibition of mitochondrial cholesterol side chain cleavage.”

“In women, AG rapidly lowers estrogen and progesterone levels in the luteal phase of the menstrual cycle and in early pregnancy, eliciting a reflex release of luteinizing hormone in the luteal phase.”

“The increase of progesterone at higher AG (300-3000 microM) concentrations could be the result of the fact that degradation is inhibited more than progesterone is formed.”

“By contrast, the delta 4-steroids such as progesterone , androstenedione, and 17 alpha- hydroxyprogesterone rose 2- to 10-fold during the initial 2 weeks of AG treatment and then fell back to starting levels or were suppressed.”

“ Aminoglutethimide was used to block progesterone synthesis.”

“Addition of aminoglutethimide , an inhibitor of cholesterol side chain cleavage (P450scc) enzyme, not only resulted in a drastic inhibition of progesterone production but also in an attenuation of StAR expression in response to either hCG or 8-Br-cAMP.”

“This relationship was observed in the presence of aminoglutethimide , where progesterone concentrations were 3 ng/ml or less.”

“Nevertheless, LH and antioxidant-induced progesterone stimulation could be blocked by the addition of either aminoglutethimide or ketoconazole, cytochrome P450 side-chain cleavage (cytochrome P450 SCC) enzyme inhibitors, which prevent the conversion of cholesterol to pregnenolone and thus block steroid hormone synthesis.”

“Acute treatment with aminoglutethimide during the mid-luteal phase decreased serum progesterone levels and increased luteal vitamin E and cholesterol ester levels.”