Possible Interaction: Amiloride and Choline

“ Choline uptake in the cells was inhibited by unlabeled choline and hemicholinium-3 as well as various organic cations including guanidine, amiloride and acetylcholine.”

“3 min(-1); 5) inhibited by the thiamine … by unrelated organic cations tetraethylammonium, N-methylnicotinamide, and choline ; and … by amiloride with an inhibition constant of 0.2 mM. The role of specific protein kinase-mediated … uptake by Caco-2 cells was also examined using specific modulators of these pathways.”

“Several commonly prescribed pharmaceuticals known to cross the placental barrier including imipramine, verapamil, propranolol, quinine, flurazepam, amiloride and ritodrin were observed to inhibit conductive choline uptake suggesting an interaction with the mechanism mediating conductive choline transport.”

“ Amiloride (0.33 mM), an inhibitor of the Na(+)‐H+ exchanger, or replacement of external sodium [Na+]o with choline induced a 60% inhibition of the recovery rate, indicating a predominant involvement of this antiporter in the response to intracellular acidification.”

“ Amiloride at concentrations of 1 mmol/L completely inhibited operation of the exchanger, as did sodium substitution with choline or tetramethylammonium.”