Possible Interaction: Alprostadil and Epinephrine

“ Prostaglandin E1 , which stimulates adenylate cyclase to form cAMP, inhibited the antibody effects 50% at 50-fold the concentration required for equivalent inhibition of aggregation and release by epinephrine.”

“ PGE1 potentiates contractions caused by other agents such as epinephrine.”

“ Prostaglandin E1 treatment weakened SC reduction in response to ADP and epinephrine (SC of 8.8 ± 1.8% and 9.5 ± 2.0%, respectively).”

“Ca(2+)-mobilizing effects of lipoproteins were potentiated about 4-fold by epinephrine and completely blocked by prostaglandin E1 both in platelets of healthy and HFH subjects.”

“We found that: (i) ticlopidine inhibits aggregation by ADP but … by Adr of ADP-induced aggregation; (ii) ADP, Adr or thrombin … raised by PGE1 , an effect inhibited by ticlopidine only for ADP and not for Adr or … (iii) Ca2+ influx and Ca2+ mobilization from internal stores were not affected.”

“In intact platelets, PMA, like PGE1, stimulated the return of the calcium transient to resting values after a thrombin stimulus, but only the PGE1 effect was reversed by adrenaline.”

“Pretreatment of the PRP with epinephrine increased the maximum transmittance during ADP aggregation, produced less-reversible aggregates (less subject to deaggregation by PGE1 ), and increased the aggregate flow resistance over the range of filtration pressures tested.”

“The inhibition of ADP-induced aggregation and rise in [Ca2+]i is potentiated by PGE1 which stimulates adenylate cyclase and is inhibited by adrenaline which inhibits adenylate cyclase.”

“ Adrenaline , high thrombin concentrations and, in the absence of phosphocreatine and creatine kinase, ADP inhibited cyclic [3H]AMP formation induced by PGE1.”

“Coupling to adenylate cyclase was also assessed in separate studies of the inhibition by adrenaline of PGE1 stimulated cyclic AMP accumulation in whole platelets.”