Possible Interaction: Alkaline Phosphatase and Vitamin C

“ AA increased the activity of alkaline phosphatase and the expression of the alkaline phosphatase gene Akp2, markers of osteoblast differentiation, in MC3T3 pre-osteoblasts cultured with LNCaP or LNShh cells.”

“In view of the potentiating effect of ascorbic acid on rhBMP-2-induced stimulation of alkaline phosphatase , we speculate that ascorbic acid could amplify the osteoinductive effects of rhBMP-2 and thereby augment the efficacy of the BMP when used as bone repair material in vivo.”

“Ascorbic acid AA ) enhances osteoblastic differentiation by increasing collagen accumulation, which in turn, results in increased alkaline phosphatase (AP) expression in some osteogenic cells.”

“As a kind of hydrolysis enzyme, ALP can promote the hydrolysis of l-ascorbic acid 2-phosphate to ascorbic acid (AA).”

“However, ascorbic acid AA ), the product of the hydrolysis of 2-phosphate-l-ascorbic acid trisodium salt (AAP) in the presence of ALP , can effectively inhibit the self-polymerization of dopamine (DA) to form a PDA layer.”

“The presence … ACP or ALP dephosphorylates the substrate ascorbic acid 2-phosphate to produce ascorbic acid, which then reduces Cu(BCDS)22- into Cu(BCDS)23- , leading to a turn-on spectral absorption at 484 nm and a dramatic color change of the solution from colorless to orange-red.”

“ ALP catalyzes the hydrolysis of ascorbic acid phosphate and generates ascorbic acid, which is measured chronoamperometrically.”

“A combination of ascorbic acid and type I collagen remarkably upregulated Runx2, ALP , and OCN gene expression and ALP activity.”

“ AsA significantly increased ALP activity after 4 d, and this activation was inhibited by the CK2 inhibitors, 2-dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole and 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazimidazole.”

“CONCLUSIONS Our findings indicated that as orbic acid in creases the ALP ac tivity of PDL cells via type I collagen production and also enhances the expression of alpha2beta1 integrin, which is a major receptor of type I collagen.”