Possible Interaction: Alkaline Phosphatase and Tretinoin

“ALP activity tests showed that ATRA or Ad-VEGF could enhance ALP activity in MEFs ( P<0.05), and ATRA had a stronger effect than Ad-VEGF; and the combined use of ATRA and Ad-VEGF significantly enhanced the ALP activity in MEFs ( P<0.05).”

“Results showed that ATRA enhanced the potential of BMP9 to induce the osteogenic markers, such as alkaline phosphates ( ALP ) and mineralization; but retinoic acid receptor a (RARa) inhibitor showed the reversal effects.”

“ Alkaline phosphatase and bone gamma carboxyglutamate protein (Bglap, Osteocalcin) were drastically reduced in RA treated cells and RA also reduced the protein levels of Runx2 and Osterix, key transcription factors for progression to a mature osteoblast.”

“ RA stimulated activity and expression of alkaline phosphatase (ALP) and upregulated activity of the ALP gene promoter.”

“In vitro, RA increased the NaB-induced increase in ALP activity.”

“After treatment with RA (10(-6) M) for 4 d, there was a significant increase in the ALP activity of HPDL cells.”

“Treatment of IEC-6 cells with all-trans retinoic acid (RA) increased the levels of activity, protein and mRNA of L/B/K ALP , whereas enterocyte-specific proteins, including intestinal ALP, sucrase-isomaltase and glucose transporter-2, were not induced.”

“This was confirmed by nuclear run-on assays, which demonstrated a 2.5-fold increase in the abundance of ALP transcripts after 6 h of RA treatment.”

“RK in the presence of ATRA increased alkaline phosphatase ( ALP ) activity in a dose-dependent manner.”

“ RA significantly upregulated gene expression and the activity of alkaline phosphatase in SHED and PDLSC.”