Possible Interaction: Alkaline Phosphatase and Melatonin

“ Melatonin markedly reduced calcium deposition and ALP activity.”

“Mechanistically, by activating melatonin receptor 2 (MT2), melatonin upregulates the gene expression of alkaline phosphatase (ALP), bone morphogenetic protein 2 (BMP2), BMP6, osteocalcin, and osteoprotegerin to promote osteogenesis while inhibiting the receptor activator of NF‐kB ligand (RANKL) pathway to suppress osteolysis.”

“ Melatonin stimulated the proliferation and alkaline phosphatase activity of human osteoblasts in a dose‐dependent manner at the pharmacological concentrations.”

“RT-PCR … that melatonin significantly up-regulated the gene expressions of … of hypertrophic chondrocytes, including type X collagen, alkaline phosphatase , runt-related transcription factor 2, Indian hedgehog and parathyroid hormone-related protein receptor, and melatonin down-regulated the mRNA expression of hallmarks of chondrocytes, including parathyroid hormone-related protein.”

“Treatment with MEL significantly enhanced Runx2 expression, ALP activity and mineralization staining.”

“ Melatonin … ALP activity, GAG synthesis and upregulated the expression of genes involved in osteogenic and chondrogenic differentiation including, ALP, osteopontin, osteocalcin, runt-related transcription factor 2, …, collagen type X, aggrecan and sex-determining region Y-box 9 in the normal control hMSCs, but did not affect the AIS groups.”

“In the control GPCs, melatonin at the concentrations of 1, 100 nM and 10 µM significantly reduced the population of viable cells, and the mRNA level of COLX and ALP compared to the vehicle.”

“After exposed to IL‐1β for 21 days, 1 μm melatonin treatment significantly increased the levels of type I collagen, ALP , and osteocalcin, and 100 μm melatonin treatment yielded the highest level of osteopontin.”

“Treatment of cultured …−4 mol/l melatonin significantly increased the gene …-regulated the activity of alkaline phosphatase and lactate dehydrogenase, two enzymes that promote de-differentiation in gastric tissue; and there was a … of endocan expression and the activities of differentiation marker enzymes in the melatonin treated cancer cells.”

“Meanwhile, melatonin up‐regulated several markers of osteoblast differentiation, including alkaline phosphatase (110%), osteopontin (218%), and osteocalcin (310%).”