Possible Interaction: Alkaline Phosphatase and Kaempferol

“In SaOS-2 osteoblasts, treatment of kaempferol increased early and late osteogenic parameters significantly, including alkaline phosphatase ( ALP ) activity, collagen synthesis, and mRNA expression levels of Runx2, osterix, osteopontin and bone sialoprotein.”

“BackgroundPrevious research revealed that luteolin could improve the activation of alkaline phosphatase (ALP) and osteocalcin in mouse osteoblasts.”

“Furthermore, treatment with Kae in rat BMSCs enhanced mineralization, elevated ALP activity, increased the expression levels of Runx-2 and Osterix and increased the levels of p-S6K and decreased the levels of p-4E/BP1 and, consistent with its ability to promote osteoblast differentiation.”

“Similarly, kaempferol induced greater activation of alkaline phosphatase activity than control cells, and it enhanced the expression of chondrogenic marker genes, such as collagen type I, collagen type X, OCN, Runx2, and Sox9.”

“In addition, treatment with kaempferol resulted in a significant elevation of alkaline phosphatase (ALP) activity, collagen content, and mineralization in the cells.”

“ Luteolin (1 μM) caused a significant elevation of collagen content, alkaline phosphatase (ALP) activity, and osteocalcin secretion in the cells (P< 0.05).”

“Cyclosporin A, verapamil, octreotide, kaempferol , daunomycin and genistein produced a concentration-dependent increase in ALP activity.”

“However, baicalein and luteolin inhibited ALP activity and flavone showed no effect.”