Possible Interaction: Alkaline Phosphatase and Edetic Acid

“We found that the endogenous alkaline phosphatase in human peripheral blood mononuclear cells was incompletely inhibited by EDTA or L-cysteine.”

“Such thermostability of human placental ALP almost disappeared after treatment with EDTA , suggesting that the divalent metal ions were required for the thermostability.”

“ AP is functionally blocked by ethylenediaminetetraacetic acid (EDTA), which allows the repeated use of AP conjugates in immunohistochemistry with different substrates.”

“MVM alkaline phosphatase activity was shown to be inhibitable in a dose- and time-dependent manner by EDTA present in the storage buffer.”

“ Alkaline phosphatases from different tissues show different susceptibilities to the irreversible inactivation by EDTA.”

“Alkaline phosphatase activity and transport of Pi in brush border membrane vesicles were also compared in the presence of EDTA or Zn2+ at concentrations which inhibit alkaline phosphatase activity.”

“Na2 EDTA creates, on the other hand, poor preservation of alkaline phosphatase probably due to the fact that this chelate contrary to the other chelates removes the essential metal from the protein, leaving an unstable enzyme molecule which undergoes denaturation.”

“Most metabolites and enzymes did not show any variation, but significant decreases in electrolytes, alkaline phosphatase , acetylcholinesterase, bile acids, fructosamine and albumin were found when EDTA was used.”

“Irreversible inhibition of alkaline phosphatase by EDTA at concentrations of 1.0mM and above has been observed.”

“This pI change was inhibited by EDTA , an inhibitor of alkaline phosphatase.”