Possible Interaction: Alkaline Phosphatase and Calcium Phosphate

“As the results shown, BCP pre-incubated in SBF and BSA-SBF up-regulated ALP activity and osteogenic related genes and proteins, which testified the positive effect of SBF and BSA-SBF.”

“iPSC-MSCs on CPC in osteogenic medium had 10-fold increase in ALP protein than that in control medium (p<0.05).”

“All ALP activity on CPC composites was significantly higher when compared with the 10.0 +/- 3.3 of tissue culture polystyrene (p < 0.05).”

“ ALP hydrolyses inorganic pyrophosphate, which is a strong inhibitor of calcium phosphate deposition.”

“Alkaline phosphatase ALP ) activity and mRNA expression of various osteogenic markers increased significantly when cells were co‐cultured on materials with calcium phosphate scaffolds compared to tissue culture polystyrene or when MSCs were cultured alone.”

“Increasing ALP concentration increased amounts of CaP as well as stiffness.”

“RT-PCR for type I collagen, alkaline phosphatase, and osteocalcin studies were also carried out, and was found that the CaP enhances gene expression of ALP and OC and thus the differentiation of osteoblast-like cells.”

“The addition of calcium phosphate glass as in HC3 scaffolds increased the ALP and OC levels of MG63 cells.”

“The results demonstrate that ALP accelerates formation of thermosensitive chitosan/β-GP hydrogels and induces their mineralization with CaP , which paves the way for applications as injectable bone replacement materials.”

“ Calcium phosphate enrichment on grade 4 titanium significantly increased the messenger RNA levels for alkaline phosphatase and osteocalcin.”