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“ PROB and DIAZ at five times the molar albumin concentration also caused a decrease in CEF affinity from 4.5 x 10(4) M-1 (control) to 0.45 x 10(4) (PROB) and 3.0 x 10(4) (DIAZ) M-1 in isolated human serum albumin.”
“Equilibrium dialysis studies demonstrated that probenecid displaced MTX from albumin , increasing the effective free concentration of MTX in the incubation medium, and hence the rate of MTX influx.”