Possible Interaction: Albumin and Insulin

“BACKGROUND Insulin regulates albumin synthesis in vitro and in various experimental models.”

“ Insulin treatment reduced Amadori modification of albumin (23.2%, P < 0.02), fibrin (34.6%, P < 0.001), Ig heavy chain constant region (20.7%, P < 0.05), transferrin (25.4%, P < 0.04), and Ig light chain (13%, P < 0.02).”

“ Insulin promoted albumin uptake and the expression of amnionless and cubilin in HKC cells (P<0.05).”

“We also demonstrated insulin (100 nM) induced albumin internalization in human proximal tubule cells (hPT) and this effect of insulin was attenuated in hydroxy‐2‐naphthalenylmethylphosphonic acid (100 µM), a tyrosine kinase inhibitor, pretreated hPT.”

“The effects of Det and Deg, but not insulin and Gla were suppressed by addition of albumin.”

“If insulin was injected together with albumin prepared from the serum of diabetic patients, this effect of insulin was antagonized.”

“These observations confirm, underin vivo conditions, that diabetic albumin is more antagonistic to insulin than albumin prepared from normal subjects.”

“When Dowex treated (nonantagonistic) albumin was dissolved in water and re-extracted by the TCA-ethanol method, the resulting albumin preparations were high in chlorine content and highly antagonistic toward insulin.”

“In the present study it is shown that the affinity of fatty acid acylated insulins for albumin varies considerably (> 50-fold) among species.”

“Differences in affinity of the acylated insulins for albumin are reflected in the relative timing of the blood-glucose-lowering effect after subcutaneous injection into rabbits.”

“We conclude that the biochemical concept of albumin binding can be applied to protract the effect of insulin , and suggest that derivatization with albumin-binding ligands could be generally applicable to prolong the action profile of peptide drugs.”