Possible Interaction: Alanine and Tyrosine

“l-DOPA and l-tyrosine were the most effective competitive inhibitors of l-alanine transport, with IC50 values of 272.2 ± 57.1 and 716.3 ± 112.4 μm, respectively.”

“In addition, we have identified two putative transmembrane domain residues, phenylalanine 184 in transmembrane domain 2 and tyrosine 239 in transmembrane domain 3, for which substitution by alanine reduces the affinity of L-168,049 46- and 4.5-fold, respectively.”

“Mutating both tyrosines in the Y16/Y28 pair to alanine slowed the formation of light-scattering aggregates.”

“Competition between tyrosine and alanine for transport is shown to exist, probably between LAT1 and LAT2 isoforms.”

“Furthermore, mutation of a tyrosine motif to alanine in the transmembrane segment of 11β-HSD1 significantly reducedV max.”

“Substitution of alanine for any of two tyrosine or central valine residues markedly increased IC50, thereby indicating that these residues are critical for the activity.”

“The replacement of tyrosine 164 with alanine substantially impaired viral particle production.”

“Most notably, substitution of tyrosine 85 or phenylalanine 86 by alanine drastically reduces the affinity of p11 for annexin II, whereas replacement of these residues by tryptophan has no or only a marginal effect.”

“In particular, the branched chain amino acids (valine, iso-leucine, leucine) and alanine were markedly reduced, whereas the S-containing amino acids (taurine, cystine, methionine) and the aromatic (phenylalanine, tyrosine , trytophan, histidine) and glutamine/glutamate were increased, unchanged or only moderately reduced.”

“Immunoprecipitation assays revealed that replacement of all four tyrosines with alanines substantially reduced the ability of gK to interact with UL37.”