“In EL1, conversion of Tyr93 to Ala resulted in more than 20-fold decrease in affinity for MeTRH.”
“In EL2 and the top portion of the …, conversion of Tyr181 to Phe, Tyr188 to Ala , and Phe199 to Ala resulted in … ( > 100-fold) decrease in affinity for MeTRH , and conversion of Tyr 188 to Phe and Phe196 to … an agonist-specific 4- to 5-fold decrease in affinity.”
“In EL3, conversion of Asn289 to Ala and of Ser290 to Ala caused a large ( > 100-fold) decrease in affinity for MeTRH.”