Possible Interaction: Alanine and Serine

“Mutating serine 575 to an alanine blocked STIM1 phosphorylation, reduced basal STIM1 mobility, and rendered STIM1 insensitive to E2.”

“Significance A common variant of histidine-rich Ca-binding protein (HRC), where an alanine replaces a serine at amino acid 96, can increase the risk of dying from severe heart disease.”

“Mutation of Ser to Ala reduced the strength of interaction with the chemical transition state specifically, as shown by vanadate-ADP and beryllium fluoride-ADP trapping experiments.”

“Mutation of serine to other amino acids with varying side chains: alanine , methionine, leucine, aspartic acid, asparagine, and arginine also resulted in significant activation, indicating a serine-specific inhibitory effect.”

“Substitution of serine 60 with alanine reduces incorporation of (32)P-orthophosphate substantially.”

“Substitution of the serines by alanine impeded the access of MAL to GEMs and changed its distribution from a perinuclear distribution to an ER pattern.”

“Substitution of one Ala by Ser in the N-terminal flank had pronounced effect and peptide A2SA-75-81-A4 proved to be more effective than the native 20-mer sequence in the hybridoma as well as in the LN cell proliferation assays.”

“Simultaneous mutation of these four serines to alanines decreases the rate of agonist-induced uncoupling and the rate of agonist-induced internalization.”

“Mutational analysis of three serine residues upstream of the di-leucine motif revealed that mutation of serine 139 to an alanine reduces the initial internalization rate by 50%.”

“Mutation of this serine residue to an alanine prevented chondroitin sulfate chain addition to the core protein.”