Possible Interaction: Alanine and Lysine

“In particular, residue 120 (… IFN-alpha2; Lys in IFN-alpha2/alpha1) appears to … a "hot-spot" residue: substitution by alanine significantly decreased biological activity, and the charge-reversal mutation of residue 120 to Glu caused … loss of antiviral and antiproliferative activity for both IFN-alpha2 and IFN-alpha2/alpha1.”

“Substitution of lysine 300 with alanine in the ATPase domain P-loop abolishes both ATP hydrolysis and binding.”

“We found that a … amino acid substitution of alanine by phenylalanine … H71 or lysine by isoleucine at H93, significantly reduced the binding affinity for the TAG72 antigen by 12 and 20-fold, respectively, whereas the substitution of lysine by alanine at H73 reduced the binding affinity only two-fold.”

“Perfusion with 10 mm leucine, lysine , and histidine increased the rates of release of both glutamine and alanine.”

“Mutating lysine to alanine or to arginine at Lys588 and Lys591 of Nrf2 resulted in decreased Nrf2-dependent gene transcription and abrogated the transcription-activating effect of CREB-binding protein.”

“Alteration of lysine 220 to alanine , histidine, or arginine significantly impaired enzyme function.”

“Replacement of each Lys in P332 by Ala markedly decreased the activity.”

“In the presence of Na+, both cationic and some neutral (His, Cys, Ala , Leu, Phe) amino acids inhibited L-lysine uptake.”

“A 20-fold excess of unlabeled leucine, isoleucine, methionine, valine, alanine, lysine , histidine, phenylalanine, and glutamine inhibited uptake of leucine and phenylalanine, and six of these amino acids inhibited uptake of alanine , by more than 50% both in the presence and absence of a potassium ion gradient.”

“The reduced potency of heteropolymers of Lys (spaced with Ala or Tyr) suggests that a maximal effective distance also exists.”