Possible Interaction: Alanine and Cysteine

“Substitution of alanine for lysine, tryptophan, and cysteine reduced the peptide affinity ∼ 1- to 2.4-fold, whereas replacing leucine completely abolished binding.”

“Replacement of the two cysteins with alanines in its active site results in loss of both enzymatic activities.”

“Since the replacement of the two cysteines (Cys97 and Cys100) in CD3-ε by alanines strongly inhibited pair formation, the existence of a Cys-X-X-Cys motif involved in protein-protein interactions is suggested.”

“Mitogenic activity is increased an additional 2.6 fold by substitution of cysteine-1 with alanine.”

“Substitution of the cysteine at position 580 by alanine resulted in impairment of interchain disulfide bridge formation; the mutagenized enzyme (C580A) was secreted from recombinant cells in the monomeric form and failed to assemble into dimers.”

“Proliferation of gastric cancer cells was promoted by cysteine , but inhibited by alanine and glutamic acid.”

“We also established that phenylarsine oxide-mediated inhibition of Top1 cleavage involved the two vicinal cysteines 504 and 505, as this effect was suppressed when cysteines were mutated to alanines.”

“Substitution of cysteine 49 with alanine resulted in a semiactivated mutant enzyme with enzymatic properties partly resembling the activated form of microsomal glutathione transferase.”

“N‐Acetyl‐l‐cysteine (AC), alanine , β‐alanine, cysteine sulphinic acid (CSA), γ‐aminobutyric acid (GABA),d‐phenylalanine, phenylalanine, serine and taurine inhibited MeHg– cysteine uptake at 5°C.”

“ Cysteine inhibited it more efficiently than histidine, methionine, tryptophan, tyrosine or alanine under the conditions used.”