Possible Interaction: Alanine and Aspartic Acid

“Glutamic acid and aspartic acid increased several-fold less and alanine several-fold more than would be expected from their dietary concentrations.”

“Substitution … Ala of … Asp residue in the membraneproximal segment of the subunit or an Arg residue in the corresponding region of the subunit causes constitutive integrin activation, suggesting that a salt bridge between the side chains of the two amino acids stabilizes the inactive conformation (Hughes et al. 1996).”

“Substitution of the conserved aspartic acid at position 338 (TM VII) with alanine caused a loss of detectable receptor-evoked inhibition of cAMP generation, although this mutation did not appreciably affect ligand binding.”

“Exchange of aspartic acid at position 286 or 288 with alanine or asparagine decreased glucosyltransferase activity by about 5000-fold and completely blocked glucohydrolase activity.”

“Replacement of Arg or Asp by their stereoisomers or Gly by Ala resulted in a 100-1000-fold reduction in activity.”

“Replacement of Asp with Ala , Glu, or Asn resulted in a greater than 100-fold reduction in cleavage activity.”

“Conversely, for the L-isomer , a slight under-proportionality was detected in exposure with increasing doses of D ,L-MPH.”

“Whereas the folding of the mutated proteins was not seriously disrupted as judged by assembly into …, Golgi localization, and secretion of a soluble form of the enzyme, exchange of the highly conserved aspartic acid residues at position 356 … 358 with alanine or asparagine reduced enzyme activity to background levels.”

“Substitution of aspartic acid 911 in the putative active site with alanine resulted in the appearance of low but reproducible activity on pNPP dephosphorylation.”

“ Asp at +1 is a negative influence, since Ala and Leu substitutions at this position increase Vmax/Km by 2.3- and 6.3-fold, respectively.”