Possible Interaction: Adenosine Triphosphate and Trypsin

“Perfusion of trypsinized tachyzoites with 1 mM of either CaCl2 or MgCl2 and 1 mM ATP increased the number of activated parasites to over 60%; on the other hand, all induced motility was inhibited or blocked by agents that chelate divalent cations.”

“In addition, trypsin digestion of tDhh1p suggests that ATP binding enhances an RNA-induced conformational change.”

“Electrospray ionization mass spectrometry (ESI-LC/MS) of tryptic digests of human alphaB-crystallin in the presence and absence of ATP identified four … within the core "alpha-crystallin" domain, Lys(82), Lys(103), Arg(116), and Arg(123), that were shielded from the action of trypsin in … of ATP.”

“Our results indicated that the proteolytic susceptibilities of trypsin and chymotrypsin were altered in the presence of ATP.”

“… ATP analogue 5'-p-fluorosulfonyl benzoyl[14C]adenosine, metabolic labeling with [35S]methionine, [3H]glucosamine, or [32P]orthophosphate, or in vitro autophosphorylation with [gamma-32P]… 15-kDa portion of the receptor that contains the sites of tyrosine phosphorylation and is degraded to small fragments in the presence of trypsin.”

“In the presence of ATP and in the complexes S1.ADP.Vi or S1.ADP.BeFx, trypsin produces two additional cleavages in the S1 heavy chain: a faster cleavage in the N-terminal region between Arg23 and Ile24, and a slower cleavage at the 50 kDa fragment.”

“In control mitochondria (initially incubated with ATP ) 0.5 mg/ml trypsin caused a decrease of MAO-A activity by 32.2 +/- 4.2%, whereas in ubiquitin-incorporated mitochondria (initially incubated with ATP + ubiquitin) reduction of MAO-A activity was significantly higher (51.4 +/- 2.5%, p < 0.02).”

“ Trypsin blocked the ATP effect but not the phospholipid effect on the KACh channel kinetics.”

“Treatment of gastric membranes with trypsin does not inhibit the ANS response to potassium but does inhibit the response to ATP.”

“Incubation of the 30 kDa protein with 5 mM ATP resulted in its resistance to V8 protease or to trypsin treated with 1-chloro-4-phenyl-3-L-toluene-p-sulphonamidobutan-2-one.”