Possible Interaction: Adenosine Triphosphate and Gossypol

“ALDH inhibitor, gossypol , also reduced ATP production in a dose dependent manner together with decrease of NADH level in NSCLC.”

“31P nuclear magnetic resonance spectra of cells exposed to low concentrations of gossypol exhibited decreased levels of ATP , markedly increased levels of pyridine nucleotides, and decreased levels of glycerylphosphocholine.”

“Furthermore, gossypol , a pan-ALDH inhibitor, markedly reduced the level of ATP.”

“ Gossypol (10 microM, 5 min), an inhibitor of endothelium-derived relaxing factor (EDRF) production and/or release, increased basal RVR by 5% and significantly inhibited the vasodilator effects of ACh and ATP but had no effect on verapamil- or GTN-induced decreases in RVR.”

“The in vivo blood pressure lowering effect of three vasodilators whose in vitro effects are endothelium-dependent (acetylcholine, substance P and adenosine triphosphate ) was reduced by 40-60% after systemic treatment of the animals with gossypol.”

“However, in the presence of gossypol the levels of ATP and FBP decreased simultaneously.”

“The selective action of gossypol in suppressing the flow responses to the endothelium-dependent compounds ACh, ATP , and SP is consistent with a vasomotor role for endothelial cells in resistance-sized vessels in vivo.”

“ Gossypol , a polyphenolic binaphthalene -dialdehyde reputed to exert contraceptive action in males, reversibly inhibits adenylate cyclase [ ATP pyrophosphate lyase (cyclizing), EC 4.6.1.1] in a concentration-dependent manner.”

“Kinetic studies using partially purified catalytic … isolated from bovine testis show that gossypol is … with ATP with an apparent Ki of 110 microM. These data suggest that gossypol inhibition of adenylate cyclase is due to direct interaction at the nucleotide-binding domain of the catalytic subunit of the enzyme.”

“At gossypol: sperm ratios above 6 nmol/10(8) spermatozoa, gossypol also decreased the concentrations of the adenine nucleotides, ATP , ADP and AMP, and this is most probably the basis for its toxic effect on spermatozoa.”

“The ATP content, either under aerobic or anaerobic condition was reduced by gossypol with the reduction threshold of ATP more sensitive in the latter.”