Possible Interaction: Adenosine Triphosphate and Genistein

“At the molecular level, genistein inhibits the activity of ATP utilizing enzymes such as: tyrosine-specific protein kinases, topoisomerase II and enzymes involved in phosphatidylinositol turnover.”

“In ghosts, ATP (1-4 mM) competitively antagonizes oestradiol, genistein and cytochalasin B (CB)-dependent inhibitions of glucose exit, (K(i(ATP/ED))=2.5+/-0.23 mM, K(i(ATP/GEN))=0.99+/-0.17 mM and K(i(ATP/CB))=0.76+/-0.08 mM).”

“ Genistein (200 microM) suppressed fMLP (1 microM) and ATP (100 microM)-induced Ca2+ mobilization, by 30-40%.”

“In the presence of ATP, genistein , like the ATP analogue adenosine 5'-[beta,gamma-imido]triphosphate (pp[NH]pA), increases cystic fibrosis transmembrane conductance regulator (CFTR) chloride currents by prolonging open times.”

“PTK inhibitors tyrphostin 51 and genistein inhibited [Ca(2+)](i) response to ATP.”

“Thus, ATP and a nonhydrolyzable ATP derivative competitively inhibit the effect of genistein on the channel.”

“ Genistein , an inhibitor of tyrosine phosphorylation, suppressed the ATP response significantly, while daidzein, an inactive analogue of genistein, had little effect on it, although both genistein or daidzein alone decreased IK.”

“Indirect immunofluorescence revealed a decrease in staining of occludin, a membrane component of the TJ, in the region of the TJ after ATP depletion, which reversed after ATP repletion; this reversal process was inhibited by genistein.”

“Moreover, we show that genistein also potentiates the stimulation of Ga,Cl by ATP.”

“The inhibitory effect of I K ATP by interferon‐α was blocked by genistein and herbimycin A, tyrosine kinase inhibitors, but was not affected by N‐(2‐metylpiperazyl)‐5‐isoquinolinesulfoamide (H‐7), an inhibitor of protein kinase C and cAMP‐dependent protein kinase.”