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“When expressed in Escherichia coli fused to a maltose-binding protein, the enzyme catalysed asymmetric cleavage of Ap4A to AMP and ATP which was inhibited at concentrations of F- as low as 3 microM. These are properties characteristic of Ap4A hydrolase (asymmetrical) (EC 3.6.1.”
“These data suggest: (i) that fluoride interacts directly and slowly with the insulin receptor, which causes inhibition of its phosphotransferase activity; (ii) … fact that fluoride inhibits phosphotransferase activity in the absence of alterations in the binding of ATP, Mn2+ or insulin, we speculate that fluoride binding … ATP to … substrates.”
“Even though the ATP concentration was independent of the growth rate under these conditions, fluoride produced a characteristic metabolic alteration in these cells.”
“When fluoride was present during the entire experimental period, HeLa cells maintained an ATP concentration only slightly below control cells; but when fluoride was added to rapidly growing cells, a rapid decrease in cellular ATP was observed.”
“Thus, fluoride induced the mitochondrial dysfunction in cardiomyocytes by damaging the structure of mitochondrial and interfering with the synthesis of ATP.”
Biological Trace Element Research • 2017 | View Paper
“Moreover, the observation that fluoride mimicked the inhibitory action of ATP suggests the activation of G proteins during purinergic receptor stimulation.”
The Journal of general physiology • 2000 | View Paper
“Inhibitors acting on the glycolytic pathway (2-deoxy-D-glucose, iodoacetamide or fluoride ) strongly decreased aerobic lactate production and ATP concentration, consistent with the belief that articular cartilage obtains its principal supply of ATP from substrate-level phosphorylation in glycolysis.”
“Exposure of cardiac or … muscle SR to fluoride in the presence of both Ca2+ and ATP resulted in concentration-dependent stimulatory effect of fluoride on Ca2+ uptake with no change in EP formation or Ca(2+)-ATPase activity, this effect diminished substantially at saturating oxalate concentration in the assay.”
“The amplitude of the ATP transient is decreasing with increasing free [Ca2+] in the range of 1 nM to 30 microM. In the presence of fluoride , the ATP transient is significantly enhanced and its declining phase is substantially retarded.”