Possible Interaction: Adenosine and Pentostatin

“The five day schedule was … studies had indicated that although pentostatin had an elimination half-… hours and could inhibit plasma adenosine deaminase activity for 24 hours, recovery of enzyme activity rapidly took place and accumulation … a toxic effect on non-replicating lymphoid cells could be increased by repeated dosing.”

“Functional studies of murine hypoxia revealed inhibition of ADA with deoxycoformycin (dCF) enhances protective responses mediated by adenosine (vascular leak and neutrophil accumulation).”

“Cordycepin but not adenosine or 29-deoxyadenosine inhibited the methylation of nuclear RNA in the presence of 29-deoxycoformycin.”

“Combinations of deoxycoformycin (l μg/ml) and either adenosine or deoxyadenosine gave growth inhibition in all three systems.”

“The combination of 2'deoxycoformycin and adenosine removed not only all antigenically detectable T cells, but also functionally detectable cells, as judged by response to mitogens.”

“The combination of 2'deoxycoformycin and adenosine was found to have opposite effects on T lymphocytes and hemopoietic progenitors, i.e., the latter were preserved whereas the growth of T lymphocytes was inhibited following prolonged (4-7 days) incubation in suspension culture.”

“Inhibition of the erythrocytic nucleoside transport system by dilazep (1 microM) or dipyridamole (10 microM), or blockade of Ado metabolism by 2'-deoxycoformycin (5 microM) plus 5-iodotubercidin (10 microM), evokes the antiaggregatory action of Ado in whole blood (IC50 congruent to 2 microM).”

“Exposure of DU.528 cells to a variety of agents induced myeloid maturation; adenosine and deoxyadenosine, in the presence of deoxycoformycin , induced expression of myeloid differentiation antigens.”

“Combinations of deoxycoformycin and adenosine or deoxyadenosine gave inhibition of 3H-thymidine incorporation in both lymphocytes and cell lines.”

“ dCF decreases this production by 75 % and provokes an accumulation of Ado , demonstrating a dephosphorylation of AMP.”

“erythro-9-(2-Hydroxy non-3-yl) adenine … a competitive inhibitor, with Ki 15 nM. Deoxycoformycin inhibits deamination … both adenosine and deoxyadenosine, with an apparent Ki of 60-90 pM. A specific antibody was developed against the purified enzyme, and a sensitive radioimmunoassay for adenosine deaminase protein is described.”