Possible Interaction: Adenosine and NAD

“Formation of adenosine from NAD+ affected UA and ATP concentrations, its main downstream molecules.”

“The addition of NAD+ to membranes prepared from WT mice resulted in faster generation of adenosine compared to membranes from CD38KO mice.”

“ Adenosine and 2-chloroadenosine, in the concentration range of 10−6–10−3 M, resulted in concentration-dependent increases in CBF and NAD reduction.”

“However, the 5′-adenine nucleotides (AMP, ADP and ATP) and NAD+ all appear to act at least partially indirectly subsequent to their hydrolysis to adenosine.”

“Adenine dinucleotides such as beta-NAD , alpha-NAD, NADP, 3-aminopyridine adenine dinucleotide, flavin adenine dinucleotide, 3',5'-and 2',5'-adenylyladenosine mimicked the inhibitory effects of adenosine and adenine nucleotides on electrically evoked contractions of the rat and mouse isolated superfused vas deferens.”

“The adenosine derivatives, adenosine , AMP, ADP, and ADP-ribose, were found to inhibit the NADase-catalyzed hydrolysis of NAD.”

“Phosphoenolpyruvate (Km = 1.6 mM), adenosine 5′-triphosphate (Km = 0.5 mM), adenosine diphosphate (Km = 1.5 mM), and adenosine 5′-monophosphate (Km = 3.0 mM) are competitive inhibitors with respect to NAD+.”

“5'-Adenylic acid can inhibit both lactic dehydrogenase and glutamic dehydro-genase; since both the phosphate and adenine moieties are necessary for good in-ibition, the 5'-adenylic acid presumably competes with DPN.”

“The synthesis of guanine from adenine riboside or ribotide occurred only in the presence of added DPN.”