Possible Interaction: Adenosine and Colforsin

“ Adenosine increased cAMP formation in cells expressing the A2A (EC(50): 0.7 microM) and A2B (EC(50): 24 microM) receptors and inhibited forskolin (0.3-3 microM)-stimulated cAMP formation in cells expressing the A1 (EC(50): 0.31 microM) and A3 receptors (EC(50): 0.29 microM).”

“The objective of this study was to determine whether the apparent agonist-independent effect of PD 81,723 observed in CHO cells stably … to the agonist binding site of the A1AdoR. The allosteric enhancer PD 81,723, the A1AdoR agonist (R)-N6-(2-phenylisopropyl)adenosine and adenosine … forskolin-stimulated ….”

“ Adenosine , NECA, (R)- and (S)-PIA, and CPA inhibited forskolin-stimulated cAMP accumulation by 30-40% in stably transfected cells; I-ABA is a partial agonist.”

“Conversely, forskolin , an enhancer of intracellular cAMP, mimicked the effect of adenosine , suggesting that the cAMP/PKA signaling pathway is involved in adenosine-mediated MMP-1 inhibition.”

“In addition, forskolin (an inducer of intracellular cAMP formation) could mimic the effect of adenosine , supporting the idea of P1-A2B receptor activation.”

“After preincubation with 100 µM forskolin , application of 300 µM adenosine caused a significant further change in Va, Isc, the transepithelial potential (Vt) and fRa.”

“Here we provide evidence that a) the Mg2+-dependent activation of adenylate cyclase seen in hirudinized PRP is mediated by adenosine, b) the adenosine produced synergizes with forskolin and with DN9693 to raise the level of cAMP in platelets.”

“Moreover, the effect of forskolin was mimicked by adenosine in a dose-dependent manner.”

“Treatment of cardiac fibroblasts with forskolin , isoproterenol, or norepinephrine increased cAMP production and extracellular levels of adenosine , and these effects were prevented by inhibition of adenylyl cyclase (2′,5′-dideoxyadenosine).”

“ Forskolin activity was also potentiated by the hydrolysis-resistant adenosine receptor agonists 5'-N-ethylcarboxamido adenosine and CGS 21680 (8.9- and 12.2-fold increase, respectively).”