Possible Interaction: Acetylcysteine and Sulforaphane

“SFN formed adducts with cysteine residues in the extracellular domain of TLR4 as confirmed by liquid chromatography-tandem mass spectrometry analysis and the inhibitory effects of SFN on oligomerization and NF-κB activation were reversed by thiol donors (DTT and N-acetyl-l-cysteine).”

“Sulforaphane also activated the mitochondrial apoptotic signaling pathway with a decrease in … membrane potential and the nuclear translocation of cytochrome c. Furthermore, sulforaphane provoked the generation of intracellular ROS; especially … ROS production was blocked by antioxidant N-acetylcysteine , both AMPK activation and growth inhibition by sulforaphane were completely abolished.”

“The inhibitory effects of SFN could be abolished by exogenously supplied GSH and by the GSH replenishing antioxidant N-acetylcysteine ( NAC).”

“Activation of the Nrf2/HO-1 system after sulforaphane treatment was suppressed by pretreatment with NAC or Ly294002, a PI3K inhibitor.”

“Nrf2 protein levels in both nuclear and whole cell lysates were increased after sulforaphane treatment and were decreased by pretreatment with NAC , actinomycin D and cycloheximide.”

“Induction of the Nrf2/HO-1 after SFN treatment was potently suppressed by pretreatment with NAC.”

“ SFN also suppressed the phosphorylation of Akt (Ser-473), thereby inhibiting hTERT phosphorylation and this effect was reversed by pretreatment with NAC.”

“In this paper, we report that the NAC conjugates of four naturally occurring ITCs, including allyl ITC, benzyl ITC (BITC), phenethyl ITC and sulforaphane , potently inhibited the growth of cells derived from both low-grade superficial and high-grade invasive human bladder cancers and drug-resistant bladder cancer cells.”

“Exposure of HT-29 cells with both SFN and an antioxidant, either NAC or GSH, completely blocked the SFN-mediated activation of these MAPK signaling cascades, regulation of cyclin D1and p21CIP1 gene expression, and G1phase cell cycle arrest.”

“The inhibitory effect of SFN on the interaction of LPS and MD2 was reversed by thiol supplementation with N-acetyl-L-cysteine or dithiothreitol showing that the inhibitory effect of SFN is dependent on its thiol-modifying activity.”

“ NAC and GSH reverse the inhibitory effects of SFN upon p65 translocation and IkappaB-alpha degradation when preincubated with this agent.”