Possible Interaction: Acetylcysteine and Nitroprusside

“Co-incubation with N-acetylcysteine , a precursor of glutathione synthesis, significantly attenuated heme oxygenase induction by SNP and SNAP, and a reduction in heme oxygenase activity was also observed when cells were preincubated with N-acetylcysteine for 16 h prior to exposure to NO donors.”

“In contrast, coronary vasodilation with SNP was significantly greater after NAC (p < 0.05).”

“IFN-gamma greatly increased LPS-mediated TNF-alpha production by human neutrophils (p < 0.01), and SNP plus NAC was found to further augment this production (p < 0.01).”

“CPT (100-500 microM) or NAC (50-200 microM) but not ENA (100 and 500 microM) also potentiated the antiaggregatory effects of sodium nitroprusside (1.0 microM) or iloprost (0.2 nM).”

“RV and NAC could also suppress SNP induced NP cell apoptosis ex vivo.”

“RV and NAC siginificantly inhibited SNP induced NP cell apoptosis, production of intracellular ROS, deline of ΔΨm as well as disruption of cytoskeletal and morphological structure, while RV did not suppress NO production.”

“These results suggest that pre-administration of a combination of antioxidant NAC ) with endothelin derived vasodilators ( sodium nitroprusside and Phosphoramidon) attenuates renal ischemia-reperfusion injury, e.g. in donor kidney for transplantation, by protecting cells against free radical damage.”

“A significant elevation of expression of mRNA … after exposure of the cells to SNP at a concentration of 0.25 mM. …-1-oxyl-3-oxide (c-PTIO), N-acetylcysteine (NAC), or ebselen (Ebs) significantly suppressed the elevations induced by … (ONOO(-)) is involved in this event as a triggering molecule.”

“An NO scavenger N-acetylcysteine ), the analogue of SNP (devoid of NO), inhibited the rate of apoptosis after SNP treatment compared with SNP treatment without scavenger, but alone did not induce apoptosis.”

“It was also shown that the thiol N-acetylcysteine (NAC ) potentiates the action of SNP in hypertensive rats, pointing to the mediation of thiols in the vasodilator action of SNP in this condition.”

“Incubation with SNP plus N-acetyl-L-cysteine (NAC) further augmented the percentage of cell death with respect to SNP used alone, and this process is seen earlier, i.e. after 24 h. Moreover, the induction of apoptosis in the presence of NAC is time- and concentration-dependent.”