Possible Interaction: Acetylcysteine and Ebselen

“In VSMCs, PDGF dose-dependently increased migration … 10 ng/mL. Pretreatment with the antioxidant N-acetyl-cysteine , the flavin-… mimetic ebselen significantly attenuated migration (PDGF alone, 5.0 ± 1.1-fold; NAC, 1.8 ± 0.… migration; and ebselen, 2.0 ± 0.5-fold migration), as did overexpression of catalase.”

“Furtherly, we found that exogenous addition of N-acetyl cysteine (NAC) completely diminished the cell damage induced by ebselen.”

“Cotreatment with ebselen (20 μM) and NAC (1 mM) inhibited E2-induced BrdU incorporation without affecting the basal levels of DNA synthesis.”

“Thus, the combination of N-acetylcysteine and Ebselen may provide a useful therapeutic tool in conditions of pulmonary toxicity associated with oxidant insult.”

“The decreases in neuronal survival and mitochondrial membrane potential induced by HNE were suppressed by … generation were also suppressed by pretreatment with NAC at 1000 microM. Although simultaneous application of ebselen (10 … NAC at 300 microM. HNE increased [Ca(2+)](i) … microM), but not by MK-801 or CNQX.”

“These results suggest that pretreatment with a low concentration of NAC dramatically potentiates the neuroprotective activity of ebselen , and that HNE-induced increase in [Ca(2+)](i) is not involved in HNE-induced neuronal death in cerebellar granule neurons.”

“Further studies revealed that depletion of cellular glutathione level by the combined action of ebselen and OGD played a … effort of ebselen and OGD, compared to that of ebselen or OGD insult alone; (2) exogenous addition of N-acetyl cysteine … ebselen and OGD; (3) supplement … damage induced by ebselen.”

“ Ebselen alone did not ameliorate diquat cytotoxicity, but in combination with either GSH (1 mM) or NAC (1 mM) it produced a significant delay in diquat-induced cytotoxicity.”

“ Ebselen in combination with either GSH (1 mM) or NAC (1 mM) was capable of reducing H2O2 in both systems.”

“Thus, the combination of Ebselen and NAC may provide an effective antidote in cases of overexposure to bipyridylium herbicides, such as diquat and paraquat.”