Possible Interaction: Acetylcysteine and Arachidonic Acid

“The thiol antioxidant NAC reduces activation of cPLA2 (assessed by isoform gel-shift and membrane translocation), production of AA in AGE-albumin–exposed neutrophils (H3 release reduced to 104±17%, P =0.94 compared with albumin-exposed neutrophils), and the AGE-augmented neutrophil respiratory burst.”

“Furthermore, … N-acetyl cysteine … arachidonic acid-induced increase in intracellular ROS and TBARS, and the activation of AP1 and NFkappaB. These results indicate that the accumulation of …-3) series elicited an intracellular oxidative stress, resulting in the activation of oxidative stress-responsive transcription factors such as AP1 and NFkappaB.”

“ NAC (200 microM) decreased the number of apoptotic nuclei in normoxia in control and arachidonic acid exposed cells.”

“Here, we show topical AFC inhibits in vivo acute inflammation induced by 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and arachidonic acid using the mouse ear model of inflammation.”

“Antioxidants such as trolox or N-acetyl cysteine prevented this synergistic toxicity of SAMe plus AA or SAMe plus BSO, respectively.”

“We further demonstrated that the free radical scavenger N-acetylcysteine blocked arachidonic acid-induced JNK activation, while H(2)O(2), a reactive oxidative molecule, activated JNK in a dose-dependent manner, providing additional support for a redox mechanism.”

“Furthermore, the neurotoxic action of AA was abolished by antioxidants butylated hydroxyanisole or N‐acetylcysteine.”

“Additional experiments supported the hypothesis that n-acetylcysteine is an oxygen radical scavenger, since it reduced or prevented the free radical-dependent cerebral arteriolar dilation normally induced by the topical application of arachidonic acid or bradykinin.”