Possible Interaction: Acetylcysteine and Aldosterone

“In vitro, exposure of podocytes to Aldo enhanced NLRP3, caspase-1, and IL-18 expressions in dose- and time-dependent manners, indicating an activation of NLRP3 inflammasome, which was significantly blocked by the mineralocorticoid receptor antagonist eplerenone or the antioxidant N-acetylcysteine.”

“Our earlier work demonstrated that aldosterone activates αENaC transcription by reducing expression of Dot1a and Af9 and by impairing Dot1a-Af9 interaction.”

“Pretreatment of donor cells with aldosterone or exposure to uEVs from PA patients enhanced the effects on extracellular ATP and αENaC expression.”

“Trophoblast cell invasion ability is stimulated when αENaC was up-regulated by aldosterone , and inhibited when ENaC was down-regulated by amiloride and αENaC specific RNAi (SiENA/ENaC).”

“U0126 and NAC significantly and NAC partly inhibited the increased AP-1 DNA binding activity induced by Aldo.”

“ Aldo treatment increased NCC and αENaC colocalization (electron microscopy) and interaction (coimmunoprecipitation).”

“ Aldosterone treatment resulted in further decrements in Af9 and Dot1a association with the αENaC promoter.”

“ Aldosterone increases tubular sodium absorption in large part by increasing αENaC transcription in collecting duct principal cells.”

“ Aldosterone induces a molecular weight shift of γENaC from 85 to 70 kDa that is necessary for the channel activation.”

“The antioxidant N-acetylcysteine (2 mmol/liter) completely inhibited the ability of aldosterone to induce any of these changes.”