Possible Interaction: Acetylcholine and Melatonin

“ Melatonin (100 and 400 nm) attenuated maximal endothelium‐dependent relaxant responses to ACH slightly by 23 ± 9 and 17 ± 9% leaving responses to direct stimulation of soluble guanylate cyclase by sodium nitroprusside unchanged.”

“Treatment with melatonin significantly increased OPN and SMP30 expression and ACh , NE, and Dop levels in the hippocampus with amelioration of the oxidative stress compared with BCCAO rats.”

“ Melatonin intake increased Ach induced aortic NO in Wistar+Iso and SHR+Iso groups, whereas there was no effect of PUFA intake.”

“Norepinephrine-induced melatonin secretion from whole pineal glands was significantly decreased by the coapplication of acetylcholine (ACh).”

“In the part of contraction recording of colonic smooth muscle, melatonin inhibited the fortified spontaneous contraction induced by WAS, and decreased the amplitude of spontaneous contraction stimulated by Ach and KCl after WAS treatment.”

“Similarly, the mean peak amplitude of contractions evoked by ACh was significantly inhibited by melatonin in a concentration-dependent manner (100, 200, and 300 microM) (P <.05).”

“ Melatonin also significantly enhanced the relaxant response to acetylcholine of the pulmonary arterial rings from normoxic rats (34.76+/-5.67% versus 53.82+/-4.736%) in the absence or presence of melatonin, respectively).”

“Analyzed as a main factor in a factorial ANOVA, age decreased and melatonin augmented the relaxing response to acetylcholine.”

“Local perfusion of melatonin (200–500 μm), in a dose‐dependent manner, disrupted the ACh rhythm in the prefrontal cortex but did not affect the circadian rhythm of locomotor activity.”

“Our results revealed that MEL has an endothelium-dependent vaso-relaxant effect and potentiated significantly the vaso-relaxant effect of acetylcholine.”

“We conclude that MEL has an endothelium dependent vasorelaxant effect and potentiates the endothelium dependent vasorelaxation induced by acetylcholine.”