Possible Interaction: Acetylcholine and Gossypol

“ Gossypol almost abolished the effects of acetylcholine and substance P on vascular resistance, but had no significant effect on the responses to prostaglandin E1 and glyceryl trinitrate.”

“2 … to acetylcholine (1 μm) were inhibited by the following … the approximate IC50 values indicated in parentheses: gossypol (1.5 μm), nordi‐hydroguairetic acid (NDGA, 5 μm), AA 861 (20 μm), phenidone (30 μm), …), BW 755C (300 μm), and piriprost (500 μm); with cirsiliol 50% inhibition was not achieved.”

“In the blood-perfused hind limb of the rabbit, two irreversible inhibitors of endothelium-dependent vasodilation gossypol and p-bromophenacyl-bromide) blocked the vasodilation induced by the endothelium-dependent agent acetylcholine , but not the response to the endothelium-independent vasodilator prostaglandin E1.(ABSTRACT TRUNCATED AT 250 WORDS)”

“Hemoglobin (Hb), gossypol , and N omega-nitro-L-arginine methyl ester (L-NAME), inhibitors for endothelium-derived relaxing factor (EDRF) in mammalian blood vessels, partially inhibited vasorelaxation induced by ANG II and ACh in fowl.”

“Collagenase, gossypol , hemoglobin and ascorbic acid have been reported to inhibit the endothelium-dependent vasodilation, inhibited increase in mucosal hemodynamics induced by vagal stimulation and acetylcholine.”

“ Gossypol (10 microM, 5 min), an inhibitor of endothelium-derived relaxing factor (EDRF) production and/or release, increased basal RVR by 5% and significantly inhibited the vasodilator effects of ACh and ATP but had no effect on verapamil- or GTN-induced decreases in RVR.”

“Responses to BAEE, BAME, L‐arginine hydrochloride, L‐alanine methylester, L‐methionine methylester, L‐lysine hydroxamate and acetylcholine (but not nitroprusside) were significantly inhibited by CHAPS (4·7 mg mL−1, 30 s) de‐endothelialization as well as pretreatment of mesentery preparations with gossypol (3 μM).”

“In myometrial strips, gossypol (1-30 microM) produced rightward displacements of the cumulative concentration-response curves to acetylcholine , bradykinin and oxytocin, accompanied by reductions in maximal responses.”

“3 Gossypol (3 μm), metyrapone (10 μm) and nordihydroguaiaretic acid, (NDGA, 30 μm), presumed inhibitors of EDRF biosynthesis, reduced or abolished the response to acetylcholine and histamine in perfused kidney and mesentery of the rat without affecting vasodilatation due to nitroprusside.”

“The in vivo blood pressure lowering effect of three vasodilators whose in vitro effects are endothelium-dependent acetylcholine , substance P and adenosine triphosphate) was reduced by 40-60% after systemic treatment of the animals with gossypol.”