Possible Interaction: Acetylcholine and Glycine

“ Glycine treatment improved this modulator effect and increased relaxation to acetylcholine.”

“…)ACh … Gly in human cerebral cortex; 2) the interaction occurs through muscarinic receptors which resemble most the M4 subtype; and 3) considering that Gly is required … glutamate receptor, the ACh-evoked Gly release may represent a linkage between cholinergic and glutamatergic transmission, two systems strongly implicated in cognitive processes.”

“There are some indications that the fast acting acetylcholine and glycine have an initial effect and then rapidly desensitise.”

“… glycine activated “basal” … activation by Cl−. Acetylcholine and glutamate activated “basal” Mg2+-ATPase to a lesser extent and did not suppress the activation of the enzyme by Cl−.… by neuromediators was decreased by blockers of the corresponding receptors (picrotoxin, strychnine, benztropine mesylate, and D-2-amino-5-phosphonovaleric acid).”

“ Glycine (1 mM) had no effect on basal ACh release, but completely blocked the light-evoked release of ACh.”

“The excitatory effect of ACh and the inhibitory effect of Gly and atropine were dose-dependent.”

“Exposure of NTS and caudate nucleus, but not hypoglossal nucleus, to glycine caused release of acetylcholine in a calcium-dependent manner that varied with concentration of glycine in the incubation medium.”

“Thus, glycine , acting at strychnine-sensitive receptors in NTS, elicits release of acetylcholine from a portion of locally synthesized neurotransmitter stores.”

“Subthreshold doses of glycine , which augmented responses to acetylcholine microinjected into NTS, either decreased or had no effect on glutamate or NMDA.”

“ Glycine (100 microM) potentiated the ACh response to 1.4 times the control, and also increased the resting Ca2+ concentration slightly.”

“ Gly at a concentration of 20 mM produced significant inhibition of exocrine secretion evoked by ACh (50 nM) or GRP (36 pM).”