Possible Interaction: Acetylcholine and Genistein

“ Genistein in vitro relaxes rat arteries by a nitric oxide dependent mechanism and enhances the dilator response to acetylcholine of atherosclerotic arteries.”

“ Genistein reduced the first contraction induced by acetylcholine chloride , but did not affect the second contraction caused by CaCl2.”

“In addition, genistein , resveratrol and 17beta-estradiol also could reduce the contractile responses of ACh and KCl, and shift their cumulative concentration-response curves rightward.”

“In calcium-free Kreb's solution containing 0.01 mmol/L egtazic acid (EGTA), genistein and resveratrol inhibited the first phasic contraction induced by acetylcholine (ACh), but did not affect the second contraction induced by CaCl(2).”

“In SHRs, after 5 weeks of treatment, genistein reduced systolic blood pressure and enhanced endothelium-dependent aortic relaxation to acetylcholine , but had no effect on the vasodilator responses to sodium nitroprusside.”

“2 In intact cells, genistein (10-100 microm) and herbimycin (3-30 microm) inhibited CA release induced by acetylcholine (ACh; 100 microm) or the nicotinic receptor stimulant 1,1-dimethyl-4-phenyl-piperazinium (DMPP; 10 microm), but did not affect CA release induced by high K+ (40 mm).”

“3 Genistein and herbimycin inhibited (45)Ca2+ uptake induced by ACh (100 microm).”

“ Genistein , daidzein, or … to acetylcholine and decreased the endothelium-dependent vasoconstrictor responses to acetylcholine in SHR, but not in WKY, and these effects were not modified by … 182,780 (7α,17β-[9[(4,4,5,5,5-pentafluoropentyl)-sulfinyl]nonyl]estra-1,3,5(10)-triene-3,17-diol).”

“Moreover, genistein restored impaired aortic relaxation to acetylcholine , lipid peroxidation, and suppressed long‐term hypotension.”

“ Genistein , an inhibitor of tyrosine kinase, markedly attenuated acetylcholine-induced calcium influx to the cells; however, wortmannin had no effect on acetylcholine-induced calcium changes.”

“The ACh currents were strongly suppressed by thapsigargin, the microsomal calcium ATPase inhibitor, and genistein , the tyrosine protein kinase inhibitor.”

“ Genistein (0.1-100 microM), in a concentration-dependent manner, suppressed the activation of the inward current by 100 microM ACh , whereas it did not significantly suppress that of the outward current (n = 6-8).”