Possible Interaction: Acetylcholine and Capsaicin

“Pretreatment with capsaicin (1 mumol/L for 20 minutes) significantly shortened the duration of vasodilator response to ACh but did not affect the initial rapid phase of ACh-induced vasodilation.”

“A RhoA kinase inhibitor reduced the relaxant effect of capsaicin at 30 microM. Capsaicin and resiniferatoxin reduced acetylcholine- and caffeine-induced transient contractions in a Ca(2+)-free, EGTA solution.”

“ Capsaicin at 30 microM for 20 min did not alter basal levels of MLC(20) phosphorylation, but abolished an increase by acetylcholine in MLC(20) phosphorylation.”

“ Capsaicin suppressed acetylcholine release from the normal rat esophageal segments evoked by vagus nerve stimulation but not that from the neonatally capsaicin-treated ones.”

“Rats treated neonatally with capsaicin or with spinal nerve ligation as adults showed a significantly decreased K+-stimulated release of [3H]-acetylcholine from dorsal horn but not ventral horn lumbar spinal cord slices.”

“When the tissue was contracted for 20 min with acetylcholine , MLC(20) phosphorylation was increased, and capsaicin reduced markedly the contraction and abolished MLC(20) phosphorylation both elicited by acetylcholine.”

“This enhancement of airway response to ACh was partially inhibited by capsaicin desensitization or bilateral vagotomy.”

“The responses of RL to intravenous injections of ACh, neurokinin (NK) A, and capsaicin were all markedly increased in CS animals; for example, ACh at the same dose of 5.06 microg/kg increased RL by 207% in the control group and by 697% (n = 8; P < 0.”

“2 Capsaicin (1 to 30 μm) suppressed dopamine release and the intracellular Ca2+ increase evoked by 100 μm ACh.”

“3 Under whole‐cell voltage‐clamp, capsaicin (1 to 30 μm) reversibly inhibited the inward current activated by ACh (30 to 300 μm).”

“ Capsaicin treatment decreased vasodilator responses to acetylcholine , but only at low acetylcholine concentrations.”

“The vasodilator response to ACh was significantly smaller in rats pretreated as neonates with capsaicin to destroy primary sensory afferents than it was in age-matched controls.”