Possible Interaction: Acetylcholine and Caffeine

“Abstract Surgically denervated muscle exhibits increased sensitivity to acetylcholine and caffeine, and the acetylcholine contracture subsequent to preincubation with caffeine is greatly enhanced.”

“ Caffeine also caused elevation of [Ca2+]i and blocked responses to ACh.”

“The effects of caffeine were mimicked by acetylcholine ; in addition, acetylcholine and caffeine each occluded the response to the other agonist.”

“Conclusions Low concentrations of caffeine directly inhibit pancreatic ductal fluid secretion stimulated by secretin or ACh and also ethanol-induced fluid hypersecretion.”

“Results Both secretin-stimulated and acetylcholine (ACh)-stimulated fluid secretions were substantially and reversibly inhibited by relatively low concentrations of caffeine as low as 0.03 mM relevant to blood levels after ingestion of caffeine-containing beverages.”

“ Caffeine and kolanut consumption reduced the relaxation response to both acetylcholine and sodium nitroprusside.”

“By comparing the influence of aminophylline we can conclude, that caffeine as well as theophylline caused significantly stronger decrease of the reactivity to acetylcholine than aminophylline only in lower concentration (Fig. 4, Ref.”

“ Caffeine abolished the contractile effects of 5-HT, ACH , and KCl.”

“ Caffeine or vanadate, each at a lower concentration than the threshold for causing contraction by itself, augmented the contractile responses of the trachea to acetylcholine (1 μmol/l–0.3 mmol/l).”

“Again carbachol was found not to influence the Ca metabolism.2.In the presence of elevated [Ca++]0 (8 mM) or of caffeine (10−3–5·10−3 M) the ACh induced contractures were potentiated and their time courses were prolonged.”

“If the … or caffeine , which itself did not … in the presence of ACh , a sustained increase in tension was induced and the time course of twitch contractions was considerably prolonged.3.… ACh or caffeine nor the addition of calcium to the bathing medium were able to produce a contracture.”

“In intact fibre bundles, 10 μm acetylcholine induced a tonic contraction which had a magnitude of 65.4±4.0 N cm-… mm) produced a force response which was 157.1±7.9% (n=16) of the acetylcholine response at 23° C and acetylcholine … caffeine together produced … caffeine alone.”