“E. coli metabolism depended on dilution rate; at high dilution rates, above 0.4 h−1, the methionine addition enhanced β‐lactamase production and reduced acetic acid formation , while at low dilution rates, below 0.3 h −1, the effect was reversed.”
“In the presence … g/L of acetic acid , addition of 0.5 … of glycine or methionine resulted in either a complete recovery or a further enhancement in the specific growth rate, while the … was significant but not fully complete in the presence of 4 g/L of acetic acid.”
“The addition of 0.5 g/L of methionine alleviated the negative effect of acetic acid on recombinant E. Coli growth to produce more β‐lactamase, which was encoded by plasmid pUC18.”